Urofollitropin products: preparation process explained

　　The invention discloses a method for preparing urofollitropin. The preparation method has the advantages of simple operation and low production cost, and the obtained urofollitropin has higher purity and no foreign protein pollution. The specific activity of FSH is greater than 10000IU/mg protein, and the titer of LH:FSH is lower than 1:100. The preparation method comprises the following steps:

　　(1) The crude product of human menopausal gonadotropin is purified by anion exchange chromatography to obtain Urofollitropin intermediate I;

　　(2) Urofollitropin intermediate I is purified by cation exchange chromatography to obtain Urofollitropin intermediate II;

　　(3) Urofollitropin intermediate II is purified by dye affinity chromatography to obtain Urofollitropin intermediate III;

　　(4) Urofollitropin intermediate III is purified by hydrophobic chromatography to obtain a solution of high-purity Urofollitropin;