The preparation method for preparing urofollitropin has the advantages of
simple operation, low production cost, higher purity of the obtained
urofollitropin, and no pollution by exogenous protein. The specific activity of
FSH is greater than 10000IU/mg protein, and the LH: FSH titer is lower than
1:100. The preparation method includes the following steps:
(1) The crude product of human menopausal gonadotropin is purified by anion
exchange layer Chemicalbook analysis to obtain urinary follicle-stimulating
hormone intermediate I;
(2) urofollitropin intermediate I am purified by cation exchange
chromatography to obtain urofollitropin intermediate II;
(3) UFO intermediate II is purified by dye affinity chromatography to
obtain UFO intermediate III;
(4) urofollitropin intermediate III is purified by hydrophobic
chromatography to obtain a solution of high-purity urofollitropin;
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