Preparation process of urofollitropin: a preparation method of urofollicle
stimulating hormone, the preparation method is easy to operate, low in
production cost, and the obtained urofollitropin has higher purity and no
contamination by exogenous protein. The specific activity of FSH is greater than
10000IU/mg protein, and the LH:FSH titer is lower than 1:100. The preparation
method includes the following steps:
(1) human menopausal gonadotropin crude product is purified by anion
exchange chromatography to obtain urinary follicle stimulating hormone
intermediate I;
(2) urofollitropin intermediate I is purified by cation exchange
chromatography to obtain urofollitropin intermediate II;
(3) UFO intermediate II is purified by dye affinity chromatography to
obtain UFO intermediate III;
(4) urofollitropin intermediate III is purified by hydrophobic
chromatography to obtain a solution of high-purity urofollitropin;
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