Preparation method of urofollitropin

　　The preparation method of Urofollitropin has the advantages of simple operation, low production cost, higher purity of the obtained urofollitropin, and no pollution by exogenous protein. The specific activity of FSH is greater than 10000IU/mg protein, and the LH:FSH titer is lower than 1:100. 

      The preparation method comprises the following steps: (1) the crude product of human menopausal gonadotropin is purified by anion exchange chromatography Chemicalbook to obtain the urofollicle stimulating hormone intermediate I; (2) the urofollitropin intermediate I is purified by cation exchange chromatography to obtain the urofollicle stimulating hormone Intermediate II; (3) UFO intermediate II is purified by dye affinity chromatography to obtain UFO intermediate III; (4) UFO intermediate III is purified by hydrophobic chromatography to obtain a high-purity solution of UFO;

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