Urovollitropin is a preparation method of urofollitropin, the preparation
method is easy to operate, low in production cost, and the obtained
urofollitropin has higher purity and no contamination by exogenous proteins. The
specific activity of FSH is greater than 10000IU/mg protein, and the LH:FSH
titer is lower than 1:100.
The preparation method includes the following steps:
(1) The crude product of human menopausal gonadotropin is purified by anion
exchange chromatography Chemicalbook to obtain urinary follicle stimulating
hormone intermediate I;
(2) urofollitropin intermediate I is purified by cation exchange
chromatography to obtain urofollitropin intermediate II;
(3) UFO intermediate II is purified by dye affinity chromatography to
obtain UFO intermediate III;
(4) urofollitropin intermediate III is purified by hydrophobic
chromatography to obtain a solution of high-purity urofollitropin;
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