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Product preparation of Urofollitropin manufacturer

  Urovollitropin is a preparation method of urofollitropin, the preparation method is easy to operate, low in production cost, and the obtained urofollitropin has higher purity and no contamination by exogenous proteins. The specific activity of FSH is greater than 10000IU/mg protein, and the LH:FSH titer is lower than 1:100.

  The preparation method includes the following steps:

  (1) The crude product of human menopausal gonadotropin is purified by anion exchange chromatography Chemicalbook to obtain urinary follicle stimulating hormone intermediate I;

Product preparation of Urofollitropin manufacturer

  (2) urofollitropin intermediate I is purified by cation exchange chromatography to obtain urofollitropin intermediate II;

  (3) UFO intermediate II is purified by dye affinity chromatography to obtain UFO intermediate III;

  (4) urofollitropin intermediate III is purified by hydrophobic chromatography to obtain a solution of high-purity urofollitropin;

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