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Patent us7022822b1 discloses a preparation method of high-purity urotropin.
The process is that the crude urotropin is purified by cation exchange
chromatography, then by anion exchange chromatography, and finally by
hydrophobic chromatography. Due to the great difference in hydrophobic
properties between FSH and LH, strong elution conditions need to be used to
purify both at the same time, such as the addition of ethanol. This elution
condition will elute the impurity protein with hydrophobicity between FSH and
LH. Therefore, the specific activity of the final product is only about 4000iu /
mg protein. To prepare higher purity urotropin, it is necessary to develop and
use more effective separation technology to purify FSH and LH at the same time
to achieve the final 1:1 ratio, and remove impurity proteins as much as
possible.
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