Urokinase supplier specializes in the design, production and supply of
high-quality biological processing and purification equipment, such as urokinase
extraction equipment, gonadotropin production equipment, etc. Kangyuan is a
professional manufacturer of urokinase. The following is to explain the analysis
of the basic refining process of urokinase products, the following specific
1.Collection: After the bucket is filled with urine, store it in a cool
place. Adjust urine PH to below 6.5. In order to prevent the deterioration of
urine in summer, 0.8 phenol was added.
2, precipitation treatment: urine to keep below 10℃, with sodium hydroxide
PH value to 8.5, mix evenly, stand for 1 hour, after precipitation with a rubber
pipe siphon out the supernatant, acidification to PH value 5-5.5.
3, diatomite adsorption. According to the proportion of acidified urine
volume L, pre-cleaned and processed into neutral diatomite, which was adsorbed
at 5℃ for hours.
4. Elution. First, the diatomite adsorbent was washed once with 5℃ cold
water, and then loaded into the diatomite column, washed with 0.02% ammonia, so
that the washing solution changed from muddy to clear, and then washed with 0.02
ammonia and 0.1m sodium chloride solution immediately. When the water flowing
from the diatomite column becomes clear and muddy, it begins to collect
The above is kangyuan xiaobian for you to sort out and share the product
refining process, I hope to help you understand the product; Later continue to
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