Urokinase crude products must through introducing the before everybody is
not strange, the benefits and the market is very considerable, because this has
the very important medicinal value, so now also has studied the extraction
methods of many products, at present there are mainly three kinds of extraction
methods are respectively foaming method, precipitant method and adsorbent, and
what of the concrete operation process?
The production process of urokinase crude product is described as
1. Collect urine: male urine is collected by special plastic buckets and
the freshness of urine relationship with yield and quality is bigger, urine
urokinase protease in acidic conditions, hematuria and female urine contains the
intestines powder, such as red blood cells affect the yield and quality, so only
use male urine as raw material, urine is best stored in below 10 degrees Celsius
to deal with as soon as possible, rapid cooling.
2. Adsorption urokinase: the supernatant of the siphon will be adjusted
with hydrochloric acid plus 1% into the resin, stirring adsorption 1 hour after
the stop stirring, the resin naturally sink, siphon, remove the residual urine,
collect the resin.
3. Washing and cleaning: wash the collected resin with tap water for three
times, and then wash it with ionized-free water for three times until the urine
is colorless, and then remove the supernatant.
4. Elution: for the first elution, eluent was stirred with 3.5% volume for
elution for one hour, and the second eluent was eluted with the same eluent for
30 minutes. The eluent was collected by pumping and the volume was measured by
combining two eluents.
5. Ammonium sulphate precipitation: add solid ammonium sulphate in
accordance with the eluent, and when it reaches a certain saturation, collect
brown urokinase through paper pulp filtration for precipitation, and then drain
it and store it in freezer freezer.
The last step is to regenerate the resin and wash it with hydrochloric acid
for one hour until it is neutral.
The production process of urokinase crude products is introduced here
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