Urokinase crude is an important raw material for finishing urokinase, which
has high production technology and large market demand. Kangyuan Company has a
professional technical team and production and processing workshop, specializing
in the production and supply of urokinase and other production raw materials.
Today, our company will briefly explain the processing and extraction process of
urokinase crude products to you.
1.Collection: After filling the bucket with urine, store it in a cool
place. Adjust the urine PH until below 6.5. Urine is easy to deteriorate in
summer, and 0.8 phenol is added to prevent deterioration. Each collection of
urine should be processed within 8 hours. Precipitation treatment: keep urine
below 10℃, adjust PH value to 8.5 with sodium hydroxide, mix evenly, stand for 1
hour, after precipitation, siphon out supernatant with rubber hose, acidify to
PH value 5-5.5. Diatomite adsorption, according to the proportion of urine
volume l after acidification, adding pre-cleaning treatment into neutral
diatomite, adsorption at 5℃.
2, elution. First, the diatomite adsorbent was washed once with cool water
at 5℃, then loaded into the diatomite column and washed with ammonia water to
make the washing solution clear from muddy, and then washed with ammonia water
and sodium chloride solution. Urokinase is collected when the water flowing from
the diatomite column turns muddy from clear.
The above crude urokinase processing and extraction process, I hope to help
you understand the product; We will continue to share with you in the future.
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