Urokinase is an enzyme protein isolated from the urine of healthy people or
obtained from human kidney tissue culture. It is composed of protein with
molecular weight of 33000 mg and 54000 mg, respectively. This product direct
effects on endogenous fibrinolysis system, which can catalytic cracking
fibrinolytic enzyme into a fibrinolytic enzyme, which can not only degradation
of fibrin clot, also can degrade fibrinogen in blood, and clotting factor Ⅴ and
blood coagulation factor Ⅷ etc, so as to play a role of thrombolysis.
Methods of urokinase extraction:
1. Fill the pail with urine and store it in a cool place. Adjust the PH of
urine to below 6.5. In summer, the urine is easy to go bad, so 0.8 phenol is
added to prevent wei. Each collection of urine should be disposed of within 8
2. Precipitation treatment: keep the urine below 10℃, adjust the PH value
to 8.5 with sodium hydroxide, mix it evenly, let it stand for L hours, after
precipitation, siphon out the supernatant with rubber tube, and acidify it to
the PH value of 5-5.5.
3. Adsorption of diatomite. According to the proportion of urine volume L
after acidification, pre-cleaned diatomite was added and adsorbed at 5℃ for
4. The elution. The diatomite adsorbents are washed once in 5℃ cold water,
and then loaded into the diatomite column (column ratio 1). 1) Wash with 0.02%
ammonia to make the washing liquid clear from muddy, then wash immediately with
0.02 ammonia and 0.1m sodium chloride solution. Urokinases are collected when
the water from the diatomite column is muddied.